Abstract
Background: Blackricebrancontainsbioactivecompounds;previousstudieshaveshowntheethanolextractofblackricebran (EEBRB) can decrease levels in diabetic rats. Aims and Objective: To elucidate the mechanism of antidiabetic effect of EEBRB. Materials and Methods: This research was carried out by setting a few parameters for antidiabetic. The effects of inhibition of the enzyme a-glucosidase performed with in vitro studies using the extracted enzyme of intestines of mice. A total of 5 mL a-glucosidaseenzymewasincubatedfor5minwithEEBRB1mLwithaserialconcentrationinphosphatebufferpH7.2.Enzyme inhibitory effect was determined by measuring the concentration of formed glucose with Lane–Eynon method. The insulin level and glucose-lowering activity were determined on rats induced by alloxan 150 mg/kg and treated with EEBRB. Treated rats for 28 days were sacriï¬ced and were prepared for histopathological observation of pancreas.Result: EEBRB was able to inhibit the activityoftheenzymea-glucosidaseonglucosewithIC valuesof121 + 12.3mg%.TheEEBRBextractdoseof100mg/kgwas 50 ± able to decrease blood sugar levels to 151 38.58 mg/dL. The level of insulin in diabetic rats administered by EEBRB dose of ± ± ± 50, 100, and 200 mg/kg was obtained 6.52 5.94, 11.5 3.5, and 15.20 9.5 ng/mL, respectively. Meanwhile, histopathological observations showed an improvement on the pancreatic beta cells. Conclusion: Antidiabetic mechanism of EEBRB is associated with increased insulin levels because of regeneration of pancreatic beta cells in diabetic rats.