Abstract
                 Background: Sterilization and disinfection in hospitals is a significant concern for both the medical and the general   community. There has been an increase in many infectious diseases such as acquired immunodeficiency syndrome  (AIDS) and hepatitis B because of inadequate sterilization. Objective: To know the knowledge, attitude, and practice regarding sterilization among health-care staff working in   a tertiary hospital. Materials and Methods: This descriptive, observational hospital-based cross-sectional study was conducted from   October 2013 to December 2013. The study participants included final-year medical students, interns, nursing staff,   laboratory technicians, ward boys, and sanitation staff working in the institute who deal with patients, blood, etc. The study  was conducted using pretested, semi-structured pro forma. The data were tabulated and interpretation was done using  percentages through EpiInfo software, version 3.5.1. Result: The study included 280 participants. Of them, 254 participants returned completed questionnaires. The  respondents  included 111 final-year students and interns, 83 nurses, 11 laboratory technicians, and 49 sanitary staff.   Majority (61%) of the respondents belonged to the age group of 21-30 years. More than two-third of study participants  had been working in the hospital since the last 1–5 years. Only 44% respondents received training for sterilization and its   management. As per the respondents, AIDS (74.4%) and hepatitis B (55.9%) were the main infectious diseases transmitted   due to inadequate sterilization. Conclusion: There was inadequate knowledge among health-care staff regarding sterilization. They need to be trained  on a regular basis to improve not just their knowledge but also attitude and practice.